Mass Spectrom., 22 (2008)Īmmonium acetate in water, 50 mM pH adjusted to 4.5 with formic acid Heller, FDA Center for Veterinary Medicine, Laurel, MD 20708 USAĭavid N. Separation of Cyanuric Acid Melamine Food Contaminants GradientĪ: 95% acetonitrile in 0.1% aqueous formic acidī: 50% acetonitrile in 20 mM aqueous ammonium formateĭavid N. Learn more about melamine analysis in the pharmaceutical industry. Application ExamplesĪmong numerous other applications, the use of SeQuant® ZIC®-HILIC columns has been recommended by the US FDA (United States Food and Drug Administration) in its methods for the analysis of melamine ad related potential contaminants. Furthermore, HILIC is easy to combine with a wide range of detection methods, and boasts improved sensitivity with some of these techniques, such as electrospray ionization mass spectrometry (ESI-MS). This results in more efficient separation. Thus, it unites the benefits of aqueous mobile phases with separation based on normal phase principles in which more polar compounds respond with higher retention. Simply put, HILIC is a normal-phase type of separation but uses reversed-phase type eluents. HILIC or hydrophilic interaction liquid chromatography is a high-performance liquid chromatographic (HPLC) technique for separation of polar and hydrophilic compounds. Kawachi et al., Journal of Chromatography A, Vol. “In particular, the zwitterionic phase ZIC®-HILIC showed better selectivity for the test samples than the other phases.” Their conclusion was that the selectivity of SeQuant® ZIC®-HILIC was higher and more versatile than that of all other stationary phases. Independent Comparison of HILIC ColumnsĪ group of distinguished Japanese scientists performed comprehensive screening to compare the performance of 15 different HILIC columns. Alternative choices for separation of polar hydrophilic compounds include SeQuant® ZIC®-cHILIC with complementary selectivity, and SeQuant® ZIC®-pHILIC for higher pH stability. The columns have been used in numerous methods for a variety of compounds, such as acids and bases, anions and cations, carbohydrates, metabolites, metal complexes, amino acids, peptides, protein digests and many more. Its favorable retention and robust performance have made SeQuant® ZIC®-HILIC one of the most popular HILIC columns throughout the world. All columns are available with 3 or 5 µm particles, and pore sizes of 100 or 200 Å. The comprehensive SeQuant® ZIC®-HILIC product range includes classical analytical and capillary columns, as well as semi-preparative formats. Separation is achieved by hydrophilic partitioning combined with weak ionic interactions resulting in maximum selectivity, high loadability and easy optimization of methods. These columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. ![]() SeQuant® ZIC®-HILIC HPLC columns have documented excellent selectivity for separations of polar hydrophilic compounds in HPLC and LC-MS methods. Excellent Separations of Polar Hydrophilic Compounds
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